Premium
Genetics of somatic mammalian cells. IX. Quantitation of mutagenesis by physical and chemical agents
Author(s) -
Kao FaTen,
Puck Theodore T.
Publication year - 1969
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1040740305
Subject(s) - mutagenesis , chinese hamster , methylnitronitrosoguanidine , ethyl methanesulfonate , chinese hamster ovary cell , mutagen , methyl methanesulfonate , mutant , biology , somatic cell , microbiology and biotechnology , mutation , mutation frequency , genetics , gene , carcinogen , gene mutation , ethylnitrosourea , cell culture
The system previously described for inducing single gene mutations in Chinese hamster cells has been extended to produce additional auxotrophic mutants. An improved method for quantitating the efficiency of single gene mutation to specific auxotrophies has been developed. Mutagenesis in the forward direction has been measured after treatment of these cells with ethyl methanesulfonate, N‐methyl‐N 1 ‐nitro‐N‐nitrosoguanidine, hydroxylamine, an acridine mustard (ICR‐191), caffeine and ultraviolet‐ and X‐irradiation. For each agent, the single cell survival curve and the efficiency of chromatid breakage and rearrangement were measured. Similar measurements were also carried out with a water‐soluble carcinogen N‐nitrosomethylurea, which was shown to be effective in producing auxotrophic, somatic mutations. These results offer promise of illuminating the relationships between cell killing, chromosomal aberration, single gene mutations and carcinogenesis produced by various agents. The methods described can be used in routine testing of drugs, food additives, and environmental pollutants for mutagenic action in mammalian cells in vitro .