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Differentiation of a cell line of myeloid leukemia
Author(s) -
Ichikawa Yasuo
Publication year - 1969
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1040740303
Subject(s) - cell culture , myeloid leukemia , clone (java method) , in vitro , cellular differentiation , myeloid , agar , biology , strain (injury) , leukemia , microbiology and biotechnology , cell , immunology , chemistry , biochemistry , genetics , anatomy , gene , bacteria
A cell line was established in vitro from a spontaneous myeloid leukemia of SL strain mice. This cell line was cytologically identified as myeloblast, and its normal differentiation appeared to be completely blocked in mass culture. When the line cells were seeded in soft agar with a conditioned medium from normal cells, either macrophages or neutrophil granulocytes appeared from a single clone. The rate of formation of colonies containing differentiated cells always increased with an increase in the concentration of conditioned medium. The conditioned medium from this line cell was not as effective as was that from normal cells in inducing differentiation.