Incorporation of H 3 ‐uridine and the isolation and characterization of RNA from squid axon | Zendy

Fischer S. | Zendy; Gariglio P. | Zendy; Tarifeño E. | Zendy

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Incorporation of H 3 ‐uridine and the isolation and characterization of RNA from squid axon

Author(s) -

Fischer S.,

Gariglio P.,

Tarifeño E.

Publication year - 1969

Publication title -

journal of cellular physiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.529

H-Index - 174

eISSN - 1097-4652

pISSN - 0021-9541

DOI - 10.1002/jcp.1040740207

Subject(s) - axoplasm , squid , squid giant axon , uridine , axon , rna , loligo , biochemistry , microinjection , biophysics , biology , chemistry , microbiology and biotechnology , ecology , gene

H 3 ‐Uridine microinjected in the giant axons of the squid is incorporated in a TCA insoluble material. There is no difference between stimulated and resting axons as to the amount incorporated. The amount incorporated is increased if the stimulation precedes the microinjection of the tracer. RNA was purified and characterized from the axoplasm, axon sheaths and from a purified membrane preparation obtained from squid retinal nerve.

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