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Incorporation of H 3 ‐uridine and the isolation and characterization of RNA from squid axon
Author(s) -
Fischer S.,
Gariglio P.,
Tarifeño E.
Publication year - 1969
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1040740207
Subject(s) - axoplasm , squid , squid giant axon , uridine , axon , rna , loligo , biochemistry , microinjection , biophysics , biology , chemistry , microbiology and biotechnology , ecology , gene
Abstract H 3 ‐Uridine microinjected in the giant axons of the squid is incorporated in a TCA insoluble material. There is no difference between stimulated and resting axons as to the amount incorporated. The amount incorporated is increased if the stimulation precedes the microinjection of the tracer. RNA was purified and characterized from the axoplasm, axon sheaths and from a purified membrane preparation obtained from squid retinal nerve.