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Stimulation of mitotic activity and the initiation of deoxyribonucleic acid synthesis in populations of rat thymic lymphocytes by magnesium
Author(s) -
Whitfield J. F.,
Perris A. D.,
Rixon R. H.
Publication year - 1969
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1040740102
Subject(s) - mitosis , magnesium , extracellular , stimulation , calcium , dna synthesis , in vitro , microbiology and biotechnology , biology , biochemistry , chemistry , medicine , endocrinology , organic chemistry
The progression of rat thynocytes (maintained in vitro ) into mitosis is profoundly affected by the level of magnesium in the medium. Increasing the extracellular MgC1 2 concentration from 0 to 1.0 m M does not affect mitotic activity, but a further increase to 1.2 and 2.5 m M rapidly and strongly stimulates the flow of cells into mitosis. The level of extracellular magnesium also governs the mitogenic effectiveness of calcium ions. The mitogenic action of magnesium stems from an ability to stimulate the initiation of deoxyribonucleic acid synthesis.