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Thymidine as a synchronizing agent. II. Partial recovery of HeLa cells from unbalanced growth
Author(s) -
Lambert W. Clark,
Studzinski George P.
Publication year - 1969
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1040730310
Subject(s) - thymidine , hela , synchronizing , dna , dna synthesis , mitosis , deoxyribonuclease , cell growth , biology , cell , deoxyribonuclease i , uridine , biochemistry , rna , microbiology and biotechnology , base sequence , topology (electrical circuits) , mathematics , combinatorics , gene
Randomly growing cultures of HeLa‐S3 cells were subjected to synchronizing treatment with excess thymidine. Unbalanced growth occurred during the thymidine treatment, but the abnormal cellular composition was found to revert to control levels so that at the time of the first mitosis following a 12–24 hour treatment with the inhibitor the cellular DNA, RNA and protein content and cell size were close to control values. However, an alkaline deoxyribonuclease which attacks denatured DNA was still found to be significantly elevated at this time. The results argue against the use of thymidine as a synchrony producing agent.