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Effect of exogenous ATP on the volume of TA 3 ascites tumor cells
Author(s) -
Stewart Carleton C.,
Gasic Gabriel,
Hempling Harold G.
Publication year - 1969
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1040730206
Subject(s) - ouabain , ascites , permeability (electromagnetism) , membrane permeability , chemistry , membrane , biophysics , tumor cells , cell , nucleotide , biochemistry , microbiology and biotechnology , biology , medicine , sodium , cancer research , organic chemistry , gene
When exogenous ATP is added to suspensions of TA 3 ascites tumor cells suspended in Ca ++ and Mg ++ free media, a significant increase in cell volume can be measured. This increase is reversible upon addition of Ca ++ and/or Mg ++ back to the media. The enlargement of these cells is temperature sensitive and specific for ATP; no other nucleotides, EDTA or ouabain were effective. The evidence suggest that this phenomena may be due to an alteration in membrane permeability and that the regulation of membrane permeability is an energy dependent process.