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Recovery of cells in vitro from the effects of hypoxia and hyperoxia
Author(s) -
Shaw David H.,
Pace Donald M.
Publication year - 1969
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1040730205
Subject(s) - hyperoxia , hypoxia (environmental) , lactic acid , in vitro , metabolism , oxygen , oxygen tension , carbohydrate metabolism , cell culture , biology , cytotoxic t cell , chemistry , andrology , biochemistry , bacteria , medicine , genetics , organic chemistry
The cytotoxic effect of high, as well as low, oxygen tension of proliferation and metabolism of Low line cells in culture is reversible even after several days of exposure provided the cells are returned to 95% air + 5% CO 2 environment. This suggests that the activity of certain mechanisms within the cells may have been altered or in other ways inhibited by the abnormal environments but are quite rapidly regenerated once the adverse condition is removed. The cells tolerate a low O 2 exposure for at least 20 days while continuous exposure to high O 2 atmosphere results in degeneration and death after 7–10 days. Both glucose utilization and lactic acid production are elevated in cultures exposed to either low or high O 2 tensions, although they are markedly higher in the latter condition. When cell so exposed are returned to an air + 5% CO 2 atmosphere, rate of glucose uptake and lactic acid formation soon approaches that found in control cultures.