Optimal oxygen tension for human lymphocytes in culture | Zendy

Andersen V. | Zendy; HellungLarsen P. | Zendy; Sørensen S. F. | Zendy

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Optimal oxygen tension for human lymphocytes in culture

Author(s) -

Andersen V.,

HellungLarsen P.,

Sørensen S. F.

Publication year - 1968

Publication title -

journal of cellular physiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.529

H-Index - 174

eISSN - 1097-4652

pISSN - 0021-9541

DOI - 10.1002/jcp.1040720302

Subject(s) - phytohaemagglutinin , dna synthesis , in vitro , oxygen tension , thymidine , hyperoxia , dna replication , microbiology and biotechnology , oxygen , hypoxia (environmental) , dna , biology , cell culture , cell , chemistry , biochemistry , genetics , organic chemistry

Human lymphocytes stimulated with phytohaemagglutinin in vitro show maximal 14 C‐thymidine incorporation when the gas phase of the cultures contains 20% O 2 . The rate of DNA synthesis is more affected by hyperoxic conditions than by hypoxia. Cell viability, defined by dye exclusion, is considerably less dependent on pO 2 than is cellular replication.

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