Premium
Optimal oxygen tension for human lymphocytes in culture
Author(s) -
Andersen V.,
HellungLarsen P.,
Sørensen S. F.
Publication year - 1968
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1040720302
Subject(s) - phytohaemagglutinin , dna synthesis , in vitro , oxygen tension , thymidine , hyperoxia , dna replication , microbiology and biotechnology , oxygen , hypoxia (environmental) , dna , biology , cell culture , cell , chemistry , biochemistry , genetics , organic chemistry
Human lymphocytes stimulated with phytohaemagglutinin in vitro show maximal 14 C‐thymidine incorporation when the gas phase of the cultures contains 20% O 2 . The rate of DNA synthesis is more affected by hyperoxic conditions than by hypoxia. Cell viability, defined by dye exclusion, is considerably less dependent on pO 2 than is cellular replication.