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Enzymatic reactions at termini of DNA
Author(s) -
Hurwitz Jerard,
Becker Andrew,
Gefter M. L.,
Gold M.
Publication year - 1967
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1040700413
Subject(s) - polynucleotide , dna , enzyme , biochemistry , chemistry , phosphate , alkaline phosphatase , nucleotide , phosphatase , covalent bond , nucleic acid , rna , microbiology and biotechnology , biology , organic chemistry , gene
A number of reactions which lead to the removal of 3′‐phosphate and 5′‐phosphate ends of DNA have been detected in extracts of E. coli . Two new enzymes which attack the ends of DNA chains have been purified from phage‐infected cells. One enzyme removes only 3′‐phosphate termini specifically from DNA, whereas the other removes only 5′‐phosphate ends from both DNA and RNA. Synthetic reactions occurring at ends of DNA have also been detected. In phage‐infected extracts, 5′‐hydroxyl polynucleotide kinase catalyzes the phosphorylation of 5′‐hydroxyl‐terminated polynucleotides. Another phage‐induced enzyme, in the presence of ATP and deoxynucleoside triphosphates, leads to the conversion of 5′‐ 32 P‐labeled DNA to a form resistant to alkaline phosphatase. This enzyme preparation also catalyzes the conversion of the Hershey circle form of lambda DNA to a covalent form. Enzyme fractions purified from E. coli K12 also catalyze the latter reaction.