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A change in growth potential of cells after conversion by Rous sarcoma virus
Author(s) -
Bader John P.
Publication year - 1967
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1040700310
Subject(s) - rous sarcoma virus , virus , embryo , biology , virology , suspension (topology) , agar , microbiology and biotechnology , bacteria , genetics , mathematics , homotopy , pure mathematics
Chick embryo cells transformed by Rous sarcoma virus (RSV) were able to grow in suspension, either as colonies when trapped in nutrient agar, or in spinner cultures using liquid medium. Two strains of RSV, RSV (RAV‐1) and Schmidt‐Ruppin RSV, were able to increase the ability of chick embryo cells to grow in suspension but Rous‐associated virus (RAV‐1) and polyoma virus were not. Cells growing in suspension supported high levels of RSV production and a simple method for propagating large amounts of virus is suggested. Suspended noninfected cells, which do not grow extensively, lose their ability to be infected by RSV, suggesting that cellular divisions must be in progress for successful infection by RSV.