Premium
Nucleotide action on spontaneous electrical activity of calcium deficient nerve
Author(s) -
Kuperman Albert S.,
Okamoto Michiko,
Gallin Elaine
Publication year - 1967
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1040700305
Subject(s) - nucleotide , adenosine triphosphate , adenosine , chemistry , adenine nucleotide , calcium , antagonism , biophysics , biochemistry , adenosine monophosphate , phosphate , biology , receptor , gene , organic chemistry
In concentrations having no effect on the evoked alpha‐A fiber spike, adenosine triphosphate (ATP), adenosine monophosphate (AMP) and several other nucleotides produced antagonism of spontaneous impulses in isolated desheathed frog nerve soaked in Ca free solution. ATP was only slightly more potent than AMP, indicating that high‐energy phosphate bonds and Ca complexing are not important for stabilizing action. Furthermore, sub‐effective concentrations of Ca potentiated the stabilizing action of ATP to a minimal degree and that of AMP not at all, suggesting a direct action of the nucleotide per se rather than a Ca‐nucleotide complex. Ca 45 washout experiments showed that the nucleotides did not depress efflux of Ca from nerve axons but, in fact, caused release of Ca. It was proposed that nucleotide stabilization is associated with replacement of nucleotide lost from the excitable membrane into the Ca free medium.