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Fluctuation of alkaline phosphatase activity in synchronized heteroploid cell cultures: Effects of prednisolone
Author(s) -
Melnykovych George,
Bishop Carole F.,
Swayze Mary Ann B.
Publication year - 1967
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1040700303
Subject(s) - alkaline phosphatase , hela , prednisolone , mitosis , cell culture , colchicine , chemistry , metaphase , enzyme , vinblastine , cell , microbiology and biotechnology , biology , biochemistry , medicine , genetics , chromosome , chemotherapy , gene
In two heteroploid cell lines synchronized with thymidine double block, activity of alkaline phosphatase decreased during the 12 hour period preceding mitotic peak. A return to high values was observed during the next 12 hours of synchronous cycle. Prednisolone (11β, 17α, 21‐trihydroxy‐1,4‐pregnadiene‐3,20‐dione), when added to such cell cultures increased alkaline phosphatase activity in one of the cell lines (Henle embryonic intestine) but had the opposite effect on another line (HeLa‐S3) in which the enzyme activity was decreased. Neither effect could be demonstrated if the hormone was added at the end of S phase or if cells were arrested in metaphase by vinblastine sulfate.