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Amino acid requirements for growth of the rabbit blastocyst in vitro
Author(s) -
Daniel Joseph C.,
Krishnan R. Sivarama
Publication year - 1967
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1040700204
Subject(s) - methionine , amino acid , valine , glycine , serine , blastocyst , phenylalanine , histidine , arginine , in vitro , biochemistry , threonine , cysteine , leucine , biology , chemistry , phosphorylation , embryogenesis , enzyme , gene
Ten amino acids, namely, arginine, histidine, lysine, tryptophane, methionine, phenylalanine, leucine, valine, threonine and serine were indispensable for growth of rabbit blastocysts in vitro ; others were nonessential. Of all the essential amino acids, arginine and lysine were required in relatively high concentrations, 10 −2 M and 10 −3 M, respectively, for optimum growth. Complete omission of the non‐essential amino acids from the medium markedly reduced blastocyst growth. Interaction between serine and glycine demonstrated a partial sparing action on serine by glycine, similar to that observed between methionine and cysteine. The amino acid composition of a culture medium capable of providing continuous and consistent growth of rabbit blastocysts in vitro is described.