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Tritiated thymidine effects on DNA, RNA, and protein synthetic rates in synchronized L ‐cells
Author(s) -
Krause Margarida O.
Publication year - 1967
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1040700203
Subject(s) - thymidine , tritiated water , tritium , dna , histone , biochemistry , rna , dna synthesis , biology , chemistry , microbiology and biotechnology , gene , physics , nuclear physics
Abstract Exponentially growing L ‐cells were synchronized by the double thymidine‐block method and exposed to high specific activities of tritiated thymidine. DNA, RNA, and protein synthetic rates were measured through one cell cycle with 1‐hour pulses of the appropriate C 14 ‐labelled precursors. Equivalent doses of tritiated water were substituted for tritiated thymidine in some experiments. Total amounts of DNA and histones per nucleus were determined photometrically in Feulgen and fast‐green stained cells. It was observed that incorporated tritiated thymidine has an effect distinct from that of tritiated water and that it enhances the incorporation of the precursors at specific stages of the cell cycle, to a degree roughly proportional to the dose. Photometric data indicated an increase in DNA net synthesis and a metabolic instability of histones in the H 3 ‐thymidine‐treated cells, resulting in higher DNA:histone ratios.