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Heterolysosome formation in mouse liver
Author(s) -
Mego John L.,
McQueen J. Donald
Publication year - 1967
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1040700115
Subject(s) - incubation , albumin , incubation period , chemistry , osmotic shock , hydrolysis , thermal shock , enzyme , shock (circulatory) , formaldehyde , biochemistry , biophysics , chromatography , biology , medicine , materials science , composite material , gene
When formaldehyde‐treated 131 I‐albumin was injected into mice, the total liver radioactivity did not change significantly from 5 minutes to 60 minutes after injection. There was a progressive increase with time in the amount of radioactivity associated with liver particles which could be released by osmotic shock; the quantity of material tightly bound to particles, but not releasable by osmotic shock, did not change. At five minutes after injection the liver particles did not release acid‐soluble radioactivity into the medium when incubated at 37°. These particles contain the injected protein in osmotically releasable form not associated with proteolytic enzymes and therefore correspond to phagosomes. At 10, 30 or 60 minutes after injection, the particles degraded the protein at similar rates but the activity ceased after 90 minutes incubation when only 50 to 60% of the osmotically releasable material was hydrolyzed. This cessation of activity was shown to be due to a thermal disruption of the particles during incubation.