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The incorporation of microinjected 14 C‐amino acids into TCA insoluble fractions of the giant axon of the squid
Author(s) -
Fischer S.,
Litvak S.
Publication year - 1967
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1040700110
Subject(s) - squid giant axon , axoplasm , axon , amino acid , squid , biochemistry , differential centrifugation , biology , valine , leucine , membrane , centrifugation , biophysics , chemistry , microbiology and biotechnology , ecology
Giant squid axons were microinjected with serine, valine and leucine‐C 14 under controlled electrophysiological conditions. These amino acids are incorporated into TCA insoluble fraction in the isolated axon. This incorporation is higher in the stimulated axons as compared to non‐stimulated ones. By processing separately the axoplasm and axon sheath, it was found that the last one is responsible almost entirely for the observed incorporation. Through differential centrifugation of homogenates of microinjected axons was shown that the highest incorporation occurred in the 1500 × g sediment, which probably corresponds to membranes. The incorporation of amino acids in stimulated axons, is strongly inhibited by chloramphenicol and actinomycin D.