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Properties of mitotic cells prepared by mechanically shaking monolayer cultures of chinese hamster cells
Author(s) -
Tobey R. A.,
Anderson E. C.,
Petersen D. F.
Publication year - 1967
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1040700109
Subject(s) - mitosis , chinese hamster , monolayer , microbiology and biotechnology , hamster , mitotic index , biology , biophysics , chemistry , cell culture , biochemistry , genetics
A technique has been developed for the selective detachment of mitotic cells from monolayer cultures of Chiness hamster cells with a simple reciprocating shaking machine. Cultures prepared by the shake treatment and placed in spinner flasks are routinely obtained, in which the mitotic fraction drops from 0.95–0.05 in 19 minutes. Some properties of mitotic cells prepared by this technique are described, along with a simple procedure for producing large quantities of mitotic cells. Cells chilled immediately after collection from a series of shake treatments complete mitosis synchronously upon subsequent resuspension in warm medium.