Premium
Effects of helium group gases and nitrous oxide on hela cells
Author(s) -
Bruemmer J. H.,
Brunetti B. B.,
Schreiner H. R.
Publication year - 1967
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1040690315
Subject(s) - nitrous oxide , hela , helium , solubility , chemistry , monolayer , cytoplasm , cell , biochemistry , organic chemistry
The helium group gases and nitrous oxide at superatomospheric pressures depress multiplication of HeLa cells in monolayer cultures. The effectiveness of these gases in eliciting the pressure‐dependent response follows the order N 2 O, Xe > Kr > Ar > > Ne and He. The response correlates with lipid solubility of the gases. Depression of growth by 4.2 atm Xe is reversible after exposure for one and two days. Cultures exposed to 7.2 atm Xe show irreversible damage including cytoplasmic vacuolization. Cell attachment is strongly inhibited by Xe; 36% of the cell inoculum were not attached after 24 hours. Affinity for hydrophobic sites in the cell is suggested as determining the order of effectiveness of the gases in evoking the response.