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The relation of karyotypic change to loss of contact inhibition of division in human diploid cells after SV 40 infection
Author(s) -
Weinstein David,
Moorhead Paul S.
Publication year - 1967
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1040690313
Subject(s) - ploidy , karyotype , contact inhibition , biology , wi 38 , chromosome , cell division , virus , fibroblast , transformation (genetics) , cell , cell culture , genetics , in vitro , virology , microbiology and biotechnology , gene
Following in vitro infection of human cell cultures with simian virus 40, karyotypic analyses were performed on the earliest serial culture in which cells were released from contact inhibition of division. In these cultures of diploid fibroblast‐like cells, normal karyotypes were found in excess of the statistical expectation for the number of background dividing cells. Thus, loss of contact inhibition of cell division occurs prior to the alteration of chromosome morphology. These events are two of the prime alterations in the series of steps comprising transformation by this virus. The chromosomal changes which were present represent the first cytological alteration detectable. Their distribution in the human karyotype was examined, but was found to have no relation to any specific chromosome or chromosome group.