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Differential response of two derivatives of the BHK21 hamster cell to thymidine
Author(s) -
Tobey R. A.,
Petersen D. F.,
Anderson E. C.
Publication year - 1967
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1040690310
Subject(s) - thymidine , hamster , derivative (finance) , chinese hamster , cell culture , biology , polyoma virus , transformation (genetics) , virus , chemistry , microbiology and biotechnology , dna , virology , genetics , gene , financial economics , economics
Attempts to synchronize the BHK21 hamster cell C‐13 and its polyoma‐transformed derivative P‐183 with excess thymidine resulted in the observation that the parent cell line could be readily synchronized but the transformed derivative could not. Differences in the growth pattern indicate that excess thymidine (10 m M ) stops progress of the virus‐transformed derivative at all stages in the life cycle rather than exclusively in S. The data are suggestive but do not establish that the difference is a result of the presence of the virus genome.