Premium
Density gradient centrifugation of hemopoietic colony‐forming cells
Author(s) -
Turner R. W. A.,
Siminovitch L.,
McCulloch E. A.,
Till J. E.
Publication year - 1967
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1040690110
Subject(s) - haematopoiesis , centrifugation , buoyant density , population , biology , differential centrifugation , density gradient , microbiology and biotechnology , cell , bone marrow , bovine serum albumin , stem cell , immunology , biochemistry , virus , physics , demography , quantum mechanics , sociology
Buoyant density distributions of hemopoietic colony‐forming units (CFU) from normal mouse marrow were determined by equilibrium density gradient centrifugation in bovine serum albumin (BSA) gradients. The distributions were compared with those obtained for the total population of nucleated cells from normal mouse marrow. The buoyant density distribution for CFU was found to differ from the density distribution for the total nucleated cell population, and the portion of the total cell population with densities much less than the mean value was found to contain up to a 30‐fold greater proportion of CFU than an uncentrifuged control. These results provide a preliminary approach to the purification and characterization of normal hemopoietic colony‐forming stem cells.