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Studies on carcinogenesis by avian sarcoma viruses. V. Requirement for new DNA synthesis and for cell division
Author(s) -
Temin Howard M.
Publication year - 1967
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1040690108
Subject(s) - rous sarcoma virus , mitosis , biology , provirus , dna synthesis , virus , virology , cell division , cell cycle , embryo , retrovirus , carcinogenesis , viral transformation , dna , cell , endoreduplication , microbiology and biotechnology , genetics , cancer , gene , genome
Partially synchronized secondary cultures of chicken embryo fibroblasts were exposed to Rous sarcoma virus (RSV) at various times in the cell cycle. The initiation of normal virus production could be inhibited by treatment with excess thymidine or with cytosine arabinoside without any effect on mitosis. This result is consistent with the hypothesis that the provirus of RSV is DNA, although the virion of RSV contains only RNA. It was, also, found that treatment which prevented or interferred with the first mitosis after exposure to virus prevented the initiation of normal virus production. Later mitoses did not appear to be necessary. A corollary of this finding is that virus production is synchronized by mitosis and that the length of the latent period depends upon when in the cell cycle a cell is exposed to virus.