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Nucleolar RNA synthetic activity in chinese hamster cells in vitro and the effects of actinomycin D and nogalamycin
Author(s) -
Arrighi Frances E.
Publication year - 1967
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1040690107
Subject(s) - nucleolus , rna , chinese hamster , chromatin , microbiology and biotechnology , mitosis , biology , in vitro , cytoplasm , metaphase , anaphase , chemistry , biochemistry , dna , cell , cell cycle , chromosome , gene
RNA synthesis was studied by autoradiographic analysis using tritiated uridine incorporation in the Chinese hamster cell line Dede after a one‐minute pulse labeling period. RNA synthesis continues during all stages of interphase and mitosis except during metaphase and anaphase. Cytoplasmic RNA was apparently synthesized in the nucleus, since no grains were observed above the background level in the sample immediately following the labeling. Nucleoli synthesize their own RNA and are not reservoirs for RNA synthesized elsewhere. Both actinomycin D and nogalamycin inhibited the RNA synthetic activity of chromatin and nucleoli. However, the nucleolar synthetic activity was more susceptible to these agents than that of chromatin. Furthermore, actinomycin D was a stronger inhibitor than nogalamycin.