Extraction of an actin‐like protein from the plasmodium of a myxomycete and its interaction with myosin a from rabbit striated muscle | Zendy

Hatano Sadashi | Zendy; Oosawa Fumio | Zendy

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Extraction of an actin‐like protein from the plasmodium of a myxomycete and its interaction with myosin a from rabbit striated muscle

Author(s) -

Hatano Sadashi,

Oosawa Fumio

Publication year - 1966

Publication title -

journal of cellular physiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.529

H-Index - 174

eISSN - 1097-4652

pISSN - 0021-9541

DOI - 10.1002/jcp.1040680214

Subject(s) - physarum polycephalum , myosin , plasmodium (life cycle) , actin , atpase , skeletal muscle , physarum , biochemistry , biology , myosin atpase , chemistry , microbiology and biotechnology , biophysics , enzyme , anatomy , parasite hosting , world wide web , computer science

An actin‐like protein was obtained from the plasmodia of a myxomycete, Physarum polycephalum . It forms a complex with muscle myosin A which behaves similarly to the actomyosin from rabbit striated muscle. On the addition of ATP the complex of this protein with myosin A shows a viscosity drop at high concentrations of KCl (∼0.5 M). At low concentrations of KCl (∼0.05 M) this complex superprecipitates from solutions containing 1 mM MgCl 2 and shows Mg‐activated ATPase activity. That is, the actin‐like protein converts the ATPase of myosin A to the actomyosin type.

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