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Extraction of an actin‐like protein from the plasmodium of a myxomycete and its interaction with myosin a from rabbit striated muscle
Author(s) -
Hatano Sadashi,
Oosawa Fumio
Publication year - 1966
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1040680214
Subject(s) - physarum polycephalum , myosin , plasmodium (life cycle) , actin , atpase , skeletal muscle , physarum , biochemistry , biology , myosin atpase , chemistry , microbiology and biotechnology , biophysics , enzyme , anatomy , parasite hosting , world wide web , computer science
An actin‐like protein was obtained from the plasmodia of a myxomycete, Physarum polycephalum . It forms a complex with muscle myosin A which behaves similarly to the actomyosin from rabbit striated muscle. On the addition of ATP the complex of this protein with myosin A shows a viscosity drop at high concentrations of KCl (∼0.5 M). At low concentrations of KCl (∼0.05 M) this complex superprecipitates from solutions containing 1 mM MgCl 2 and shows Mg‐activated ATPase activity. That is, the actin‐like protein converts the ATPase of myosin A to the actomyosin type.