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Effect of temperature on the activity of ribonuclease from Tetrahymena pyriformis
Author(s) -
Lazarus Lawrence H.,
Scherbaum Otto H.
Publication year - 1966
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1040680114
Subject(s) - tetrahymena pyriformis , ribonuclease , ciliate , tetrahymena , intracellular , enzyme , rna , biology , biochemistry , chemistry , ecology , gene
Intracellular ribonuclease from the ciliate Tetrahymena pyriformis GL was purified 10‐fold. After preheating for 20 minutes at 100°C of the ribonuclease preparation 80% of its activity was lost. Preheating under the same conditions, however, in the presence of RNA, did not affect the enzyme activity. Between 0°C and 41°C the apparent activation energy was 15,600 cal per deg. per mole.