Premium
Hemoglobin functions in the blood of Bufo marinus
Author(s) -
Hall F. G.
Publication year - 1966
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1040680109
Subject(s) - bohr effect , hemoglobin , toad , oxygen , hematocrit , carbon dioxide , partial pressure , chemistry , respiratory system , respiration , bufo marinus , venous blood , biology , biochemistry , anatomy , oxygen–haemoglobin dissociation curve , endocrinology , ecology , organic chemistry
The effects of three physical‐chemical factors, temperature, hydrogen ion concentration, and partial pressure of oxygen, on the respiratory functions of blood of the toad ( Bufo marinus ) have been studied. Measurements of oxygen affinity of hemoglobin in whole blood were measured tonometrically by a method devised for small quantities of blood. At pH 7.40 and 25°C blood was found to be 50% saturated with oxygen at a partial pressure of 44 mm Hg of oxygen. The Bohr effect was measured at various temperatures and found to be about one‐half that found for mammalian blood. Carbon dioxide content of toad blood changes only slightly in the oxygenated and reduced states. Thus the “Haldane” effect parallels the small Bohr effect. Toad blood was found to have average hematocrit values of 37% for erythrocytes and average hemoglobin values of 11 gm/100 ml per cubic millimeter of blood. The respiratory functions of the blood of the toad conform to the pattern of respiratory mechanisms available for gas exchange between the environment and tissues of the organism.