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Purine pyrophosphorylase as a selective genetic marker in a mouse lymphoma, P388, in cell culture
Author(s) -
Ozer Harvey L.
Publication year - 1966
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1040680108
Subject(s) - amethopterin , hypoxanthine , enzyme , transformation (genetics) , purine , cell culture , thymidine , biology , dna , hypoxanthine guanine phosphoribosyltransferase , biochemistry , microbiology and biotechnology , lymphoma , glycine , purine metabolism , cell , gene , genetics , immunology , amino acid , methotrexate , mutant
It is possible to use the purine pyrophosphorylase in mammalian cell culture systems as a genetic marker in selecting small numbers of enzyme positive cells from large populations of pyrophosphorylase negative cells of the mouse lymphoma line P388 in medium containing amethopterin, hypoxanthine, glycine and thymidine. Conversely, it is readily possible to obtain pyrophosphorylase‐deficient cells by treatment with 8‐Azaguanine. We were unsuccessful in demonstrating DNA‐mediated transformation using DNA from enzyme positive cells incubated with cells which were enzyme negative.