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Response of cultured chick heart cells to changes in ionic environment
Author(s) -
Levinson Charles,
Green James W.
Publication year - 1966
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1040670310
Subject(s) - nucleotide , saline , phosphate , primary culture , incubation , chemically defined medium , biology , andrology , phosphate buffered saline , heart cells , biochemistry , chemistry , microbiology and biotechnology , in vitro , chromatography , myocyte , endocrinology , medicine , gene
Ventricles from 11‐day‐old chick embryonic heart were disaggregated by elastase and the component cells cultured on glass in maintenance medium containing 10 μc of P 32 . After 48 hours incubation at 37°C the medium was removed, the cells rinsed and exposed to a phosphate‐free test solution for two hours. During this period samples of the test medium were removed for counting and spectrophotometric analysis. Cells incubated in solutions lacking amino acids or vitamins or serum components lost phosphate at essentially the same rate as in the complete culture medium; furthermore such cells lost very small amounts of nucleotide materials. Cells incubated in 0.16 M NaCl lost phosphate and nucleotides rapidly; the addition of either K + or Ca +2 or Mg +2 reduced phosphate and nucleotide loss and cells in balanced saline media containing all four cations, retained phosphate and nucleotides at essentially the same level as in the complete medium. These results show that primary isolated chick heart cells can be maintained for short periods in physiological saline solutions without injury and that saline balance in short term studies is a primary factor in maintaining these cells in an uninjured state.