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Reversed potentials in isolated frog skin. II. Active transport of chloride
Author(s) -
Martin Duncan W.,
Curran Peter F.
Publication year - 1966
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1040670302
Subject(s) - potential difference , frog skin , rana , microelectrode , chloride , biophysics , chemistry , electrochemical potential , significant difference , membrane potential , grenouille , transepithelial potential difference , ion transporter , electrochemistry , anatomy , sodium , biology , salientia , biochemistry , medicine , membrane , electrode , xenopus , organic chemistry , gene
Net inward transport of Cl in the absence of an electrochemical potential difference was demonstrated in the skin of two species of frog, R. pipiens and R. esculenta under conditions of low (2 mM) Cl concentration in the bathing solutions. The electrical potential profile of skins of R. pipiens was examined with microelectrodes under conditions in which the inside solution was negative relative to the outside solution. This reversal of the normal potential difference was found to arise as a result of potential changes across the outward facing electrical barriers in the skin. The reversed potential difference appears to arise, at least in part, as a result of the inward Cl transport. The effect may be due either to electrogenic Cl transfer or to variations in internal composition of the epithelial cells arising as a result of Cl transport.