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Separation of rabbit red cells by density in a bovine serum albumin gradient and correlation of red cell density with cell age after in vivo labeling with 59 Fe
Author(s) -
Bishop Charles,
Prentice Theodore C.
Publication year - 1966
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1040670122
Subject(s) - red cell , density gradient , bovine serum albumin , rabbit (cipher) , cell , in vivo , albumin , biophysics , chemistry , serum albumin , chromatography , biology , biochemistry , medicine , physics , statistics , mathematics , microbiology and biotechnology , quantum mechanics
Rabbit red cells are separated by centrifuging them for one hour at 33,000 G in density gradient tubes of bovine serum albumin. The separation represented an equilibrium situation since rebanding experiments showed that the cells from a layer would again seek that density layer when recentrifuged in a new gradient tube. When rabbits were injected with 59 Fe, the radioactive red cells at one day were nearly all light, but these labeled cells moved into the more dense layers over the next few days. This not only shows that the separation by density is discriminating but that some red cells became dense very quickly. Bearing in mind the problems of interpreting radioactive iron data because of the possibility of reutilization, it is tentatively concluded that dense red cells are not necessarily senescent red cells since these dense cells appear to persist for the normal life span of the rabbit red cell.