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Effects of peripheral benzodiazepine receptor ligands on proliferation and differentiation of human mesenchymal stem cells
Author(s) -
Lee D.H.,
Kang S.K.,
Lee R.H.,
Ryu J.M.,
Park H.Y.,
Choi H.S.,
Bae Y.C.,
Suh K.T.,
Kim Y.K.,
Jung Jin Sup
Publication year - 2004
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.10391
Subject(s) - mesenchymal stem cell , adipose tissue , cell growth , microbiology and biotechnology , stromal cell , cellular differentiation , stem cell , chemistry , flow cytometry , cell cycle , cell , biology , immunology , cancer research , biochemistry , gene
The peripheral benzodiazepine receptor (PBR) has been known to have many functions such as a role in cell proliferation, cell differentiation, steroidogenesis, calcium flow, cellular respiration, cellular immunity, malignancy, and apoptosis. However, the presence of PBR has not been examined in mesenchymal stem cells. In this study, we demonstrated the expression of PBR in human bone marrow stromal cells (hBMSCs) and human adipose stromal cells (hATSCs) by RT‐PCR and immunocytochemistry. To determine the roles of PBR in cellular functions of human mesenchymal stem cells (hMSCs), effects of diazepam, PK11195, and Ro5‐4864 were examined. Adipose differentiation of hMSCs was decreased by high concentration of PBR ligands (50 μM), whereas it was increased by low concentrations of PBR ligands (<10 μM). PBR ligands showed a biphasic effect on glycerol‐3‐phosphate dehydrogenase (GPDH) activity. High concentration of PBR ligands (from 25 to 75 μM) inhibited proliferation of hMSCs. However, clonazepam, which does not have an affinity to PBR, did not affect adipose differentiation and proliferation of hMSCs. The PBR ligands did not induce cell death in hMSCs. PK11195 (50 μM) and Ro5‐5864 (50 μM) induced cell cycle arrest in the G 2 /M phase. These results indicate that PBR ligands play roles in adipose differentiation and proliferation of hMSCs. J. Cell. Physiol. 198: 91–99, 2004. © 2003 Wiley‐Liss, Inc.