Premium
Mouse embryo‐derived NIH3T3 fibroblasts adopt an osteoblast‐like phenotype when treated with 1α,25‐dihydroxyvitamin D 3 and dexamethasone in vitro
Author(s) -
Shui Chaoxiang,
Scutt Andrew M.
Publication year - 2002
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.10157
Subject(s) - osteopontin , osteoblast , alkaline phosphatase , in vitro , osteocalcin , retinoic acid , endocrinology , ascorbic acid , medicine , phenotype , chemistry , stimulation , dexamethasone , biology , biochemistry , enzyme , gene , food science
This study examines the capability of NIH3T3 fibroblasts to express osteoblastic markers following stimulation with a number of hormones and growth factors in vitro. Of the agents tested, 1α,25‐dihydroxyvitamin D 3 (1,25(OH) 2 D 3 ) dose‐dependently induced alkaline phosphatase (ALP) activity in NIH3T3 cells, and this effect was enhanced by the addition of dexamethasone (Dex), which when administered alone caused no detectable ALP expression. The combined use of 1,25(OH) 2 D 3 and Dex also stimulated the synthesis of osteocalcin, and osteopontin. Furthermore, cells treated with the both hormones, in the presence of β‐glycerophosphate and l ‐ascorbic acid, formed mineralized plaques, indicating an osteoblast (OB) phenotype. By contrast, the differentiation induced by 1,25(OH) 2 D 3 or 1,25(OH) 2 D 3 plus Dex was significantly antagonized by transforming growth factor‐β1 and all trans ‐retinoic acid. These data indicate that NIH3T3 cells have the potential to adopt an OB‐like phenotype and may prove to be a convenient model for studying the early events of osteogenic differentiation and the specific interactions of 1,25(OH) 2 D 3 with glucocorticoids in controlling this process in vitro. © 2002 Wiley‐Liss, Inc.