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Regulatory volume decrease is actively modulated during the cell cycle
Author(s) -
Wang Liwei,
Chen Lixin,
Zhu Linyan,
Rawle Michelle,
Nie Sihuai,
Zhang Jin,
Ping Zhong,
Kangrong Cai,
Jacob Tim J.C.
Publication year - 2002
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.10156
Subject(s) - cell cycle , mitosis , tonicity , cell , cell culture , microbiology and biotechnology , biology , dna synthesis , biophysics , chemistry , dna , biochemistry , genetics
Nasopharyngeal carcinoma cells, CNE‐2Z, when swollen by 47% hypotonic solution, exhibited a regulatory volume decrease (RVD). The RVD was inhibited by extracellular applications of the chloride channel blockers tamoxifen (30 μM; 61% inhibition), 5‐nitro‐2‐(3‐phenylpropylamino)benzoic acid (NPPB, 100 μM; 60% inhibition), and ATP (10 mM; 91% inhibition). The level and time constant of RVD varied greatly between cells. Most cells conducted an incomplete RVD, but a few had the ability to recover their volume completely. There was no obvious correlation between cell volume and RVD capacity. Flow cytometric analysis showed that highly synchronous cells were obtained by the mitotic shake‐off technique and that the cells progressed through the cell cycle synchronously when incubated in culture medium. Combined application of DNA synthesis inhibitors, thymidine and hydroxyurea arrested cells at the G1/S boundary and 87% of the cells reached S phase 4 h after being released. RVD capacity changed significantly during the cell cycle progression in cells synchronized by shake‐off technique. RVD capacity being at its highest in G1 phase and lowest in S phase. The RVD capacity in G1 (shake‐off cells sampled after 4 h of incubation), S (obtained by chemical arrest), and M cells (selected under microscope) was 73, 33, and 58%, respectively, and the time constants were 435, 769, and 2,000 sec, respectively. We conclude that RVD capacity is actively modulated in the cell cycle and RVD may play an important role in cell cycle progress. J. Cell. Physiol. 193: 110–119, 2002. © 2002 Wiley‐Liss, Inc.