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PTH‐dependent adenylyl cyclase activation in SaOS‐2 cells: Passage dependent effects on G protein interactions
Author(s) -
Gao Hong,
Bodine Peter V.N.,
Murrills Richard,
Bex Fred J.,
Bilezikian John P.,
Morris Stephen A
Publication year - 2002
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.10141
Subject(s) - cycloheximide , pertussis toxin , adenylyl cyclase , cholera toxin , forskolin , gs alpha subunit , medicine , endocrinology , chemistry , g protein , western blot , microbiology and biotechnology , protein biosynthesis , biology , receptor , biochemistry , stimulation , gene
Abstract Parathyroid hormone (PTH) sensitive adenylyl cyclase activity (ACA) in SaOS‐2 cells varies as a function of cell passage. In early passage (EP) cells (< 6), ACA in response to PTH and forskolin (FOR) was relatively low and equivalent, whereas in late passage (LP) cells (> 22), PTH exceeded FOR dependent ACA. Potential biochemical mechanisms for this passage dependent change in ACA were considered. In EP, prolonged exposure to pertussis toxin (PT) markedly enhanced ACA activity in response to PTH, Isoproterenol and Gpp(NH)p, whereas ACA in response to FOR was decreased. In contrast, the identical treatment of LP with PT diminished all ACA in response to PTH, Gpp(NH)p, and FOR. The dose dependent effects of PT on subsequent [ 32 P]ADP‐ribosylation of its substrates, GTPase activity, as well as FOR‐dependent ACA, were equivalent in EP and LP. The relative amounts of Gαi and Gαs proteins, as determined both by Western blot, PT and cholera toxin (CT) dependent [ 32 P]ADP‐ribosylation, were quantitatively similar in EP and LP. Western blot levels of Gαs and Gαi proteins were not influenced by prior exposure to PT. Both PT and CT dependent [ 32 P]ADP‐ribosylation were dose‐dependently decreased following exposure to PT. However, the PT‐dependent decline in CT‐dependent [ 32 P]ADP‐ribosylation occurred with enhanced sensitivity in LP. The protein synthesis inhibitor cycloheximide partially reversed the PT associated decrease in FOR dependent ACA in EP. In contrast, cycloheximide completely reversed the PT associated decrease in FOR and as well as PTH dependent ACA in LP. Gαs activity, revealed by cyc − reconstitution, was not altered either by cell passage or exposure to PT. The results suggest that the coupling between the components of the complex may be pivotally important in the differential responsiveness of early and late passage SaOS‐2 cells to PTH. J. Cell. Physiol. 193: 10–18, 2002. © 2002 Wiley‐Liss, Inc.