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Effect of pre‐adsorbed proteins on attachment, proliferation, and function of endothelial cells
Author(s) -
Balcells Mercedes,
Edelman Elazer R.
Publication year - 2002
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.10087
Subject(s) - fibronectin , microbiology and biotechnology , cell adhesion , laminin , adhesion , chemistry , cell growth , gelatin , cell adhesion molecule , integrin , cell , extracellular matrix , biochemistry , biology , organic chemistry
As certain proteins control cell adhesion, it has been hoped that cell transplantation and tissue engineering could be augmented by pre‐adsorption of specific proteins to biological or synthetic surfaces. The questions that remain, however, are whether such proteins can affect cell production as well as adhesion, and if so, whether in a protein‐specific manner. We examined the adhesion and the biochemical secretion of bovine aortic endothelial cells (BAEC) on tissue culture polystyrene (TCPS) discs coated with fibronectin (Fn), laminin (Ln), or gelatin. The three coating proteins nonspecifically promote sub‐confluent and post‐confluent endothelial cell production of total protein up to 2.5‐fold of the reference value. Total soluble glycosaminoglycan (GAG) production slightly increased with the different coatings only at low cell density. In contrast, Ln and Fn, not gelatin, drastically enhanced post‐confluent BAEC production of prostaglandin (PGI 2 ). However, antibody‐blockage of the α 5 integrin, constituent of the Fn receptor in BAEC, appeared to inhibit the upregulation of PGI 2 production observed on Fn‐coated surfaces. The results indicate that the cell adhesion mediators used as coating agents dictate cell biological production as well as adhesion and proliferation. J. Cell. Physiol. 191: 155–161, 2002. © 2002 Wiley‐Liss, Inc.