Open AccessHsa_circ_0005529 promotes ZEB1 expression by regulating miR‐873‐5p and enhancing proliferation, invasion, and migration in gastric cancer cell lines
Author(s) -
Su Xiaobao,
Zhou Rui,
Wu Yingjie,
Zhang Yixin,
Yu Yu,
Lu Mengxiao
Publication year - 2022
Publication title -
journal of clinical laboratory analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 50
eISSN - 1098-2825
pISSN - 0887-8013
DOI - 10.1002/jcla.24742
Subject(s) - cancer , cancer research , microrna , cancer cell , cell growth , blot , metastasis , chemistry , biology , microbiology and biotechnology , medicine , gene , biochemistry
Background Gastric cancer is a relatively common tumor. As circular RNAs (circRNAs) are documented to modulate proliferation and metastasis in various cancers, we evaluated the functions of circRNAs, in particular, hsa_circ_0005529, in gastric cancer cells. Methods Levels of hsa_circ_0005529 and miR‐873‐5p were examined by qRT‐PCR, and the presence of hsa_circ_0005529 was confirmed by RNase R treatment. CCK‐8, wound‐healing, and Transwell assays were used to assess proliferation, migration, and invasion, respectively, while Western blotting was used to determine levels of zinc finger E‐box‐binding homeobox 1 ( ZEB1 ) and dual‐luciferase reporter assays to examine relationships between hsa_circ_0005529 and miR‐873‐5p. Results hsa_circ_0005529 was strongly expressed in gastric cancer where it stimulated tumorigenic behavior. Furthermore, hsa_circ_0005529 was shown to promote ZEB1 expression by sponging miR‐873‐5p, an inhibitor of ZEB1 expression. Conclusion Our research showed that hsa_circ_0005529 promoted tumorigenic behavior in gastric cancer cells by adsorbing miR‐873‐5p to modulate ZEB1 levels. This suggests that hsa_circ_0005529 may be useful as a biomarker and target for diagnosing and treating gastric cancer.