Open AccessPhenotype, genotype, and mating type determination in oral Candida albicans isolates from pediatric patients with neutropenia
Author(s) -
Jafarian Hadis,
Gharaghani Maral,
Asnafi Ali Amin,
Hardani Amir Kamal,
ZareiMahmoudabadi Ali
Publication year - 2022
Publication title -
journal of clinical laboratory analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 50
eISSN - 1098-2825
pISSN - 0887-8013
DOI - 10.1002/jcla.24664
Subject(s) - genotype , biology , genotyping , candida albicans , microbiology and biotechnology , corpus albicans , mating type , neutropenia , phenotype , chlamydospore , virulence , agar , gene , genetics , spore , bacteria , chemotherapy
Background The most frequent species of Candida to infect and colonize patients with neutropenia is still Candida albicans . This study aimed to provide detailed information on the phenotype, genotype, and mating type of oral C. albicans isolated from neutropenic pediatric patients, and to investigate how these characteristics are related. Methods Two hundred fifty‐four oral samples from patients under 18 years old with neutropenia and malignancies were collected from January to October 2021. Samples were cultured on CHROMagar Candida . Isolates of C. albicans were identified with the germ tube test, chlamydospore production on cornmeal agar, and PCR‐RFLP. Genotyping of C. albicans isolates was carried out by amplifying the 25S rDNA gene with specific CAINT‐L and CA‐INT‐R primers. MTL a1 and MTL α1 primers were used to identify each mating type. Yeast peptone dextrose supplemented with phloxine B was used to identify different phenotypes. Results Ninety‐two (36%) patients were positive for C. albicans . The mean age of patients was 7.85. Fifty‐three (58.9%) isolates demonstrated type A, 15 (16.7%) type B, 15 (16.7%) types D/E, and 7 (7.7%) type C. Three isolates each (3.3%) were homozygous for MTL a or homozygous for MTLα . All of the MTL ‐homozygous isolates were genotype A. There was a significant correlation between patients' underlying disease and genotype ( p = 0.036). There was a significant correlation between mating type and genotype ( p = 0.000). Conclusion Most of the isolates exhibited a white phenotype, noted in the literature as the most virulent. Moreover, heterozygous strains were frequent and may play a role in Candida colonization.