Open AccessRole of IL‐10 and IL‐22 cytokines in patients with primary immune thrombocytopenia and their clinical significance
Author(s) -
Liu Qifeng,
Liu Yan
Publication year - 2022
Publication title -
journal of clinical laboratory analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 50
eISSN - 1098-2825
pISSN - 0887-8013
DOI - 10.1002/jcla.24573
Subject(s) - immunology , immune system , clinical significance , medicine , immune thrombocytopenia , interleukin 10 , primary (astronomy) , antibody , physics , astronomy
Background Immune thrombocytopenia purpura (ITP) is an autoimmune disease that leads to accelerated platelet clearance. The objective of this study was to examine the clinical role of cytokines in ITP patients and to correlate them with disease stages. Materials and Methods A total of 110 ITP patients were enrolled, including 55 with active ITP, 55 with remission ITP, and 55 with healthy controls. The enzyme‐linked immunosorbent assay technique was used to examine IL ‐10 and IL ‐22 serum levels in all subjects. Real‐time quantitative PCR was used to assess the mRNA expression of IL ‐10 and IL ‐22 in PBMC. The clinical significance of both cytokines was assessed using ROC analysis. Results IL ‐10 serum levels in active ITP patients were significantly lower than in control and remission ITP subjects ( p < 0.05). IL ‐22 serum levels were elevated in active ITP patients compared to the control and remission group ( p < 0.05). mRNA expressions of IL ‐10 and IL ‐22 in active ITP patients were also having a significant difference from than control and remission ITP group ( p < 0.05). ROC analysis showed that IL ‐10 and IL ‐22 can differentiate the ITP patients from controls. A positive correlation between serum IL ‐10 and PBMC IL ‐10 with statistical significance was observed. Similarly, the serum IL ‐22 and PBMC IL ‐22 were correlated positively with statistical significance. Conclusion IL ‐10 and IL ‐22 seem to predict the clinical course of ITP, as a significant imbalance of these cytokines was detected in active ITP patients.