Open AccessPotential of circulating lncRNA CASC2 as a biomarker in reflecting the inflammatory cytokines, multi‐organ dysfunction, disease severity, and mortality in sepsis patients
Author(s) -
Wang Rui,
Zhao Jinglin,
Wei Qi,
Wang Hao,
Zhao Chao,
Hu Caihong,
Han Yu,
Hui Zhi,
Yang Long,
Dai Qingchun,
Liu Cuicui
Publication year - 2022
Publication title -
journal of clinical laboratory analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 50
eISSN - 1098-2825
pISSN - 0887-8013
DOI - 10.1002/jcla.24569
Subject(s) - medicine , sepsis , interquartile range , sofa score , biomarker , organ dysfunction , gastroenterology , proinflammatory cytokine , interleukin 6 , systemic inflammation , inflammation , immunology , biology , biochemistry
Background Long noncoding RNA (lncRNA) cancer susceptibility candidate gene 2 (CASC2) inhibits inflammation and multi‐organ dysfunction in various ways. The present study was intended to explore the potency of blood lncRNA CASC2 as a biomarker for sepsis management. Methods Totally, 184 sepsis patients and 30 healthy controls were enrolled. The reverse transcription‐quantitative polymerase chain reaction was used to detect lncRNA CASC2 expression in peripheral blood mononuclear cell samples from the subjects. Mortality during 28 days was recorded in sepsis patients. Results LncRNA CASC2 was decreased in sepsis patients [median (interquartile range [IQR]): 0.473 (0.241–0.773)] by comparison to healthy controls [median (IQR): 1.019 (0.676–1.685)] ( p < 0.001). In sepsis patients, lncRNA CASC2 was negatively correlated with Acute Physiology and Chronic Health Evaluation II (APACHE II) ( p = 0.001), Sequential Organ Failure Assessment (SOFA) ( p < 0.001), SOFA‐respiratory system ( p = 0.010), SOFA‐coagulation ( p = 0.020), SOFA‐liver ( p = 0.019), and SOFA‐renal ( p = 0.010) scores, but was not related to SOFA‐nervous ( p = 0.466) and SOFA‐cardio vascular system ( p = 0.059) scores. Additionally, lncRNA CASC2 was negatively related to tumor necrosis factor‐α ( p = 0.024), interleukin (IL)‐1β ( p = 0.013), and IL‐17A ( p = 0.002), but was not linked to IL‐6 ( p = 0.112) or IL‐10 ( p = 0.074). Furthermore, lncRNA CASC2 was lower in sepsis deaths [median (IQR): 0.286 (0.166–0.475)] than in survivors [median (IQR): 0.534 (0.296–0.811)] ( p < 0.001). Simultaneously, Kaplan–Meier (KM) curve analysis also observed that lncRNA CASC2 was inversely related to accumulating mortality in sepsis patients ( p = 0.003). While lncRNA CASC2 could independently predict lower mortality risk. Conclusion Circulating lncRNA CASC2 inadequacy indicates the release of inflammatory cytokines, severe multi‐organ injuries, and increased mortality in sepsis patients.