Open AccessGeneration of MiRNA sponge constructs targeting multiple MiRNAs
Author(s) -
Jie Junjin,
Liu Danni,
Wang Yifan,
Wu Qiong,
Wu Tingting,
Fang Rong
Publication year - 2022
Publication title -
journal of clinical laboratory analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 50
eISSN - 1098-2825
pISSN - 0887-8013
DOI - 10.1002/jcla.24527
Subject(s) - microrna , oligonucleotide , biology , sponge , computational biology , function (biology) , rna interference , gene , microbiology and biotechnology , genetics , rna , botany
Abstract Background MicroRNAs(miRNAs) are key regulators of gene expression in plants, animals and some viruses. Hence, alteration of miRNA levels in cells or tissues is common for miRNA studies. Loss‐of‐function of miRNA can be achieved using antisense oligonucleotides, sponges and gene knockout models. Methods Here, we showed an efficient, rational and economical way to construct multi‐targeted miRNA sponges with desired copies. Four copies of miRNA sponge are used as “building‐blocks”. Results These building‐blocks, which can target same miRNA or different miRNAs, are linked together through ligation. Each time of ligation can double the number of sponge copies. Conclusions In this way, we constructed lentivirus vectors harboring sponges targeting miR‐21, miR‐31 and miR‐155 and the combination of two miRNA sponges can inhibit cancer cell growth significantly.