Open AccessAssociation of perturbation of oral bacterial with incident of Alzheimer's disease: A pilot study
Author(s) -
Taati Moghadam Majid,
Amirmozafari Nour,
Mojtahedi Ali,
Bakhshayesh Babak,
Shariati Aref,
Masjedian Jazi Faramarz
Publication year - 2022
Publication title -
journal of clinical laboratory analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 50
eISSN - 1098-2825
pISSN - 0887-8013
DOI - 10.1002/jcla.24483
Subject(s) - prevotella intermedia , aggregatibacter actinomycetemcomitans , fusobacterium nucleatum , porphyromonas gingivalis , microbiome , streptococcus mutans , oral microbiome , immunology , biology , medicine , microbiology and biotechnology , periodontitis , bacteria , bioinformatics , genetics
Objective This case‐control study was designed to compare the composition of the predominant oral bacterial microbiome in Alzheimer's disease (AD) and control group. Subject A total of 30 adult participants (15 AD and 15 healthy individuals) were entered in this study. The composition of oral bacterial microbiome was examined by quantitative real‐time polymerase chain reaction (qPCR) using bacterial 16S rDNA gene. The levels of systemic inflammatory cytokines in both groups were assessed using enzyme‐linked immunosorbent assays (ELISA). Results The loads of Porphyromonas gingivalis , Fusobacterium nucleatum , and Prevotella intermedia were significantly more abundant in the AD compared to the control group ( p < 0.05). Although Aggregatibacter actinomycetemcomitans and Streptococcus mutans were relatively frequent in the AD group, no significance difference was observed in their copy number between two groups. Although the concentrations of IL‐1, IL‐6, and TNF‐α were higher in the AD group, there was a significant difference in their levels between the two groups ( p < 0.05). Finally, there was a significant relationship between increased number of pathogenic bacteria in oral microbiome and higher concentration of cytokines in patient's blood. Conclusion Our knowledge of oral microbiome and its exact association with AD is rather limited; our study showed a significant association between changes in oral microbiome bacteria, increased inflammatory cytokines, and AD.