Open AccessExosomal circular RNA hsa_circ_0006220, and hsa_circ_0001666 as biomarkers in the diagnosis of pancreatic cancer
Author(s) -
Hong Lu,
Xu Liu,
Jin Lufei,
Xu Kaiwei,
Tang Weiwei,
Zhu Yuchao,
Qiu Xuedan,
Wang Jianhua
Publication year - 2022
Publication title -
journal of clinical laboratory analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 50
eISSN - 1098-2825
pISSN - 0887-8013
DOI - 10.1002/jcla.24447
Subject(s) - pancreatic cancer , cancer , circular rna , rna , medicine , microbiology and biotechnology , oncology , biology , genetics , gene
Background Pancreatic cancer is a highly malignant tumor of the digestive system. Objective Exosomal circular RNA can be used as a biomarker for the early diagnosis of pancreatic cancer. Methods The expression of various differentially expressed circRNAs in pancreatic cancer tissues was analyzed by gene chip, exosome expression was verified by electron microscopy and Western blotting, and the expression of exosomal circRNA in pancreatic cancer cells, tissues, and plasma were determined by quantitative reverse transcription‐polymerase chain reaction (qRT‐PCR). Results Compared with healthy controls, hsa_circ_0006220 and hsa_circ_0001666 were highly expressed in exosomes in the plasma of pancreatic cancer patients. The AUC values were 0.7817 for hsa_circ_0006220, 0.8062 for hsa_circ_0001666, and 0.884 for the combined diagnosis. In addition, clinicopathological features revealed that the expression of hsa_circ_0006220 in plasma exosomes from pancreatic cancer patients was associated with CA19‐9 levels ( p = 0.0001) and lymph node metastasis ( p = 0.0005). The expression of hsa_circ_0001666 was correlated with both tumor size ( p = 0.0157) and CA19‐9 level ( p = 0.0001). Conclusions The high expression of exosomal hsa_circ_0001666 and hsa_circ_0006220 suggests that these can be used as new biomarkers for the diagnosis and treatment of pancreatic cancer.