Open AccessThe correlation of lncRNA SNHG16 with inflammatory cytokines, adhesion molecules, disease severity, and prognosis in acute ischemic stroke patients
Author(s) -
Xie Chen,
Zhu Bin,
Gu Juxian,
Sun Muhua
Publication year - 2022
Publication title -
journal of clinical laboratory analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 50
eISSN - 1098-2825
pISSN - 0887-8013
DOI - 10.1002/jcla.24439
Subject(s) - peripheral blood mononuclear cell , medicine , tumor necrosis factor alpha , cell adhesion molecule , pathogenesis , inflammation , proinflammatory cytokine , interleukin , pathophysiology , apoptosis , gastroenterology , immunology , cytokine , biology , in vitro , biochemistry
Background Long non‐coding RNA small nucleolar RNA host gene 16 (lncRNA SNHG16) is involved in the pathogenesis of acute ischemic stroke (AIS) through the regulation of brain endothelial cell viability, inflammation, atherosclerotic plaque formation, and neural apoptosis. This study aimed to evaluate the prognostic value of lncRNA SNHG16 in AIS patients. Methods Newly diagnosed AIS patients ( N = 120) were serially recruited. Their lncRNA SNHG16 expressions in peripheral blood mononuclear cells (PBMCs) were detected by reverse transcription‐quantitative polymerase chain reaction (RT‐qPCR); serum inflammatory cytokines and adhesion molecules were determined using enzyme‐linked immunosorbent assay (ELISA). The accumulating recurrence‐free survival (RFS) and overall survival (OS) were analyzed. Moreover, controls ( N = 60) were recruited and their lncRNA SNHG16 expressions in PBMCs were detected. Results LncRNA SNHG16 was declined in AIS patients compared to controls ( p < 0.001). Moreover, lncRNA SNHG16 was not related to any comorbidities in AIS patients (all p > 0.05). Interestingly, lncRNA SNHG16 was negatively related to tumor necrosis factor alpha (TNF‐α) ( p < 0.001), interleukin 6 (IL‐6) ( p = 0.013), and intracellular cell adhesion molecule‐1 (ICAM‐1) ( p = 0.024), while positively correlated with interleukin 10 (IL‐10) ( p = 0.022) in AIS patients. Besides, lncRNA SNHG16 was inversely associated with the National Institutes of Health Stroke Scale (NIHSS) score in AIS patients ( p = 0.003). During the follow‐up period, in 14 (11.7%) patients occurred recurrence and 5 (4.2%) patients died. Unexpectedly, lncRNA SNHG16 was not associated with accumulating RFS ( p = 0.103) or OS ( p = 0.150) in AIS patients. Conclusion LncRNA SNHG16 relates to lower inflammatory cytokines, adhesion molecules, and milder disease severity, but fails to predict prognosis in AIS patients.