Open AccessAbnormal expression of histone acetylases in CD8+ T cells of patients with severe aplastic anemia
Author(s) -
Qi Weiwei,
Zhang Yu,
Wang Yachen,
Wang Huaquan,
Fu Rong,
Shao Zonghong
Publication year - 2022
Publication title -
journal of clinical laboratory analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 50
eISSN - 1098-2825
pISSN - 0887-8013
DOI - 10.1002/jcla.24339
Subject(s) - cd8 , aplastic anemia , histone , pathogenesis , medicine , endocrinology , immune system , biology , messenger rna , immunology , cancer research , gene , bone marrow , genetics
We aimed to investigate the balance between the mRNA levels of histone acetyltransferases (HATs) and histone deacetylases (HDACs) in CD8+ T cells of patients with severe aplastic anemia (SAA). Methods Twenty untreated SAA patients, 18 remission SAA patients (R‐SAA), and 22 normal controls were evaluated. The mRNA expression levels of HATs, HDACs, and IFNG in CD8+ T cells were measured by real‐time quantitative reverse transcription polymerase chain reaction. Results Histone acetylase EP300 and CREBBP mRNA levels were significantly elevated in CD8+ T cells of SAA patients compared with the normal controls (both p < 0.05). No significant differences were observed in HDAC1 and HDAC7 mRNA between SAA patients and the normal controls. There was an obvious positive correlation between IFNG and EP300 ( r = 0.5126, p < 0.01), and CREBBP ( r = 0.4663, p < 0.05), respectively, in SAA and R‐SAA patients. In addition, EP300 and CREBBP mRNA levels were clearly correlated with clinical parameters of peripheral blood and bone marrow in those patients. Conclusion Our findings suggest that EP300 and CREBBP are increased in CD8+ T cells of SAA patients and are correlated with disease severity. The imbalances in HATs and HDACs may play a role in activating CD8+ T cells to promote the immune pathogenesis of SAA.