Open AccessVirucidal effect of monogalactosyl diacylglyceride from a green microalga, Coccomyxa sp. KJ, against clinical isolates of SARS‐CoV‐2 as assessed by a plaque assay
Author(s) -
Hayashi Kyoko,
Asai Satomi,
Umezawa Kazuo,
Kakizoe Hidehumi,
Miyachi Hayato,
Morita Masanobu,
Akaike Takaaki,
Kuno Hitoshi,
Komatsu Satoko,
Watanabe Takumi,
Kawahara Toshio
Publication year - 2022
Publication title -
journal of clinical laboratory analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 50
eISSN - 1098-2825
pISSN - 0887-8013
DOI - 10.1002/jcla.24146
Subject(s) - virus quantification , virology , titer , covid-19 , virus , microbiology and biotechnology , biology , agarose , coronavirus , infectious disease (medical specialty) , medicine , disease , pathology
Background Severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) is the causative agent of coronavirus disease 2019 (COVID‐19) and is capable of human‐to‐human transmission and rapid global spread. Thus, the establishment of high‐quality viral detection and quantification methods, and the development of anti‐SARS‐CoV‐2 agents are critical. Methods Here, we present the rapid detection of infectious SARS‐CoV‐2 particles using a plaque assay with 0.5% agarose‐ME (Medium Electroosmosis) as an overlay medium. Results The plaques were capable of detecting the virus within 36–40 h post‐infection. In addition, we showed that a monogalactosyl diacylglyceride isolated from a microalga ( Coccomyxa sp. KJ) could inactivate the clinical isolates of SARS‐CoV‐2 in a time‐ and concentration‐dependent manner. Conclusions These results would allow rapid quantification of the infectious virus titers and help develop more potent virucidal agents against SARS‐CoV‐2.