Open AccessThe change of gut microbiota‐derived short‐chain fatty acids in diabetic kidney disease
Author(s) -
Zhong Chenyu,
Dai Zhiwei,
Chai Lingxiong,
Wu Lingping,
Li Jianhui,
Guo Weiying,
Zhang Jie,
Zhang Qun,
Xue Congping,
Lin Haixue,
Luo Qun,
Cai Kedan
Publication year - 2021
Publication title -
journal of clinical laboratory analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 50
eISSN - 1098-2825
pISSN - 0887-8013
DOI - 10.1002/jcla.24062
Subject(s) - renal function , butyrate , medicine , feces , propionate , diabetes mellitus , dysbiosis , gastroenterology , chemistry , type 2 diabetes , gut flora , endocrinology , urine , univariate analysis , disease , biology , biochemistry , microbiology and biotechnology , multivariate analysis , fermentation
Background Previous studies found the dysbiosis of intestinal microbiota in diabetic kidney disease (DKD), especially the decreased SCFA‐producing bacteria. We aimed to investigate the concentration of the stool and serum short‐chain fatty acids (SCFAs), gut microbiota‐derived metabolites, in individuals with DKD and reveal the correlations between SCFAs and renal function. Methods A total of 30 participants with DKD, 30 participants with type 2 diabetes mellitus (DM), and 30 normal controls (NC) in HwaMei Hospital were recruited from 1/1/2018 to 12/31/2019. Participants with DKD were divided into low estimated glomerular filtration rate (eGFR)(eGFR<60ml/min, n=14) and high eGFR (eGFR≥60ml/min, n=16) subgroups. Stool and serum were measured for SCFAs with gas chromatograph‐mass spectrometry. Results The DKD group showed markedly lower levels of fecal acetate, propionate, and butyrate versus NC ( p <0.001, p <0.001, p =0.018, respectively) [1027.32(784.21–1357.90)]vs[2064.59(1561.82–2637.44)]μg/g,[929.53(493.65–1344.26)]vs[1684.57(1110.54–2324.69)]μg/g,[851.39(409.57–1611.65)] vs[1440.74(1004.15–2594.73)]μg/g, respectively, and the lowest fecal total SCFAs concentration among the groups. DKD group also had a lower serum caproate concentration than that with diabetes ( p =0.020)[0.57(0.47–0.61)]vs[0.65(0.53–0.79)]μmol/L. In the univariate regression analysis, fecal and serum acetate correlated with eGFR (OR=1.013, p =0.072; OR=1.017, p =0.032). The correlation between serum total SCFAs and eGFR showed statistical significance (OR=1.019, p =0.024) unadjusted and a borderline significance (OR=1.024, p =0.063) when adjusted for Hb and LDL. The decrease in serum acetate and total SCFAs were found of borderline significant difference in both subgroups ( p =0.055, p =0.050). Conclusion This study provides evidence that in individuals with DKD, serum and fecal SCFAs levels (fecal level in particular) were lowered, and there was a negative correlation between SCFAs and renal function.