Open AccessDeveloping an ultra‐performance liquid chromatography‐tandem mass spectrometry for detecting aldosterone in human plasma
Author(s) -
Lin Wenbin,
Yao Zhenrong,
Li Yuzhe,
Liao Zhihao,
Xiao Jiahao,
Chen Yonghong,
Feng Pinning,
Gan Wenjia
Publication year - 2021
Publication title -
journal of clinical laboratory analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 50
eISSN - 1098-2825
pISSN - 0887-8013
DOI - 10.1002/jcla.24029
Subject(s) - chromatography , aldosterone , mass spectrometry , tandem mass spectrometry , chemistry , liquid chromatography–mass spectrometry , tandem , plasma , medicine , materials science , physics , quantum mechanics , composite material
Background Accurately measuring plasma aldosterone concentration is difficult but meaningful for primary aldosteronism (PA) diagnosis. Methods In this study, we developed an ultra‐performance liquid chromatography‐tandem mass spectrometry (UPLC‐MS/MS) method for plasma aldosterone detection, evaluated its performance according to guidelines issued by CLSI, including detection limit, linearity, precision, and compared it with chemiluminescence immunoassay. Then, a reference range of plasma aldosterone in young people was established by using this method. Results The lower limit of quantitation (LOQ) was 10 pg/ml. The mean recovery rates of analyte added to serum were 100.07–102.05% in different concentrations. The linearity range was 20–2000 pg/ml. Inter‐assay CVs were 2.20–3.97% at aldosterone concentrations of 65.66–854.75 pg/ml. The regression equation of UPLC‐MS/MS (x) and chemiluminescence immunoassay ( y ) was y = 1.002x + 65.854 ( r = 0.9456, n = 237). The reference range of plasma aldosterone detected by UPLC‐MS/MS was 11.30–363.82 pg/ml in young people in South China, and there was no statistically significant difference in plasma aldosterone concentration between two genders. Conclusion In conclusion, UPLC‐MS/MS can rapidly and accurately detect plasma aldosterone and is appropriate for clinical application.