Open AccessThe expression profile and clinical application value of hsa_circ_0016148 in head and neck squamous cell carcinoma
Author(s) -
Shen Zhisen,
Wang Liuqian,
Ye Dong
Publication year - 2021
Publication title -
journal of clinical laboratory analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 50
eISSN - 1098-2825
pISSN - 0887-8013
DOI - 10.1002/jcla.23997
Subject(s) - head and neck squamous cell carcinoma , hazard ratio , circular rna , carcinogenesis , microrna , oncology , cancer research , head and neck , medicine , microarray , confidence interval , biology , head and neck cancer , cancer , gene expression , gene , biochemistry , surgery
Background Dysregulated circular RNAs (circRNAs) are involved in human cancers and may be used as biomarkers with the potential of clinical application. However, little is known regarding the mechanism of circRNAs and their clinical application value in head and neck squamous cell carcinoma (HNSCC). Methods In the current study, we established the profile of circRNAs in HNSCC using microarray and then measured the expression of hsa_circ_0016148 in 137 paired HNSCC tissues by qRT‐PCR technique, analyzed the relationship between hsa_circ_0016148 and clinicopathological data, and investigated its diagnostic and prognostic value. The hsa_circ_0016148‐miRNA‐mRNA interaction network was predicted and constructed by Cytoscape. Results Our study showed a circRNA expression profile and confirmed downregulated hsa_circ_0016148 in HNSCC tissues ( p = 2.64E‐35). The hsa_circ_0016148 expression is remarkably correlated with lymph node metastasis ( p = 0.001) and clinical stage ( p = 0.029). Then, the area under the receiver characteristic curve (AUC) was 0.912 with 92% of sensitivity and 86.9% specificity, respectively. Besides, our study demonstrated that lower‐expressed hsa_circ_0016148 could independently predict poorer overall survival of HNSCC patients (hazard ratio [HR] = 0.456; 95% confidence interval [CI] = 0.265–0.784; p = 0.005). The hsa_circ_0016148‐miRNA‐mRNA interaction network was constructed, which included a total of nine targeted miRNAs. Conclusion Taken together, our results revealed that hsa_circ_0016148 might play a critical role in HNSCC tumorigenesis and may serve as an indicator with the potential of diagnosis and prognosis for HNSCC.