Open AccessEffects of m6A RNA methylation regulators on endometrial cancer
Author(s) -
Zhang Yanli,
Yang Yingjie
Publication year - 2021
Publication title -
journal of clinical laboratory analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 50
eISSN - 1098-2825
pISSN - 0887-8013
DOI - 10.1002/jcla.23942
Subject(s) - endometrial cancer , methylation , biology , proportional hazards model , rna methylation , rna , oncology , dna methylation , gene , gene expression , cancer research , medicine , cancer , bioinformatics , genetics , methyltransferase
Background The modification 6‐methyladenine (m 6 A) is the most common type in RNA methylation. Our study aims to explore the bioinformatic analysis of m 6 A in endometrial cancer. Methods The expression of 23 m 6 A RNA methylation regulators was compared through The Cancer Genome Atlas (TCGA) database among 406 endometrial tissue and 19 normal tissue samples. The Wilcoxon test was applied to compare the relationship between the clinicopathological characteristics and expression. Cox regressions were performed to identify the prognostic factors associated with overall survival. Gene ontology (GO) and Gene Set Enrichment Analysis (GSEA) were performed to evaluate the potential pathways. Results YTHDF2, HNRNPA2B1, HDRNPA2B1, YTHDF1, FMR1, IGF2BP3, METTL13, RBM15B, IGF2BP1, YTHDF3, YTHDC1, ZC3H13 IGF2BP2, KIAA1429, METTL14, RBMX, FTO, ALKBH5, and METTL16 were significantly abnormally expressed in endometrial cancer tissue samples. Both univariate and multivariate Cox regression analyses indicated that age, grade, and risk score were independent risk factors. High expression of FTO was associated with worse overall survival. Conclusion M 6 A RNA methylation regulators play vital role in endometrial cancer.