Open AccessEstablishment of the reference intervals of whole blood neutrophil phagocytosis by flow cytometry
Author(s) -
Yang Fangfang,
Zhang Fujie,
Yang Liping,
Li Haoran,
Zhou Yonglie
Publication year - 2021
Publication title -
journal of clinical laboratory analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 50
eISSN - 1098-2825
pISSN - 0887-8013
DOI - 10.1002/jcla.23884
Subject(s) - phagocytosis , flow cytometry , staphylococcus aureus , whole blood , microbiology and biotechnology , escherichia coli , granulocyte , cytometry , lymphocyte , immunology , neutrophile , biology , chemistry , bacteria , inflammation , biochemistry , genetics , gene
Abstract Objective To investigate the reference intervals (RIs) of the whole blood neutrophil phagocytosis by flow cytometry (FCM) and to study the application value of neutrophil phagocytosis in infectious diseases. Methods Pathogens ( Escherichia coli ATCC 25922, Staphylococcus aureus ATCC 25923) cultured for 18–24 h were labeled by fluorescence probe carboxyfluorescein diacetate succinimidyl ester (CFDA‐SE), and then incubated with whole blood at 37℃. The phagocytosis of pathogens by neutrophils was detected by flow cytometry, and a reference interval was established. Results In the healthy adults, the reference interval for the neutrophil phagocytosis to Escherichia coli was 46.91%–83.09% and to Staphylococcus aureus was 33.92%–69.48%. This method showed good reproducibility. Neutrophil phagocytosis was negatively correlated with the neutrophil count, neutrophil percentage, and neutrophil‐to‐lymphocyte ratio (NLR, p < 0.05). Conclusion We have successfully established the RIs of neutrophil phagocytosis in whole blood in healthy adults by flow cytometry (FCM), which might be of important clinical value in the diagnosis, treatment, and prognosis of infectious diseases.